Uptake of tire wear additives by vegetables grown for human consumption

Car tires contain hundreds of chemical additives that can leach out of them. This is how they end up in crops and subsequently in the food chain. Researchers at the Center for Microbiology and Environmental Systems Science at the University of Vienna and the Hebrew University of Jerusalem have now detected these chemical residues in leafy vegetables for the first time. Although the concentrations were low, the evidence was clear, a finding that is also known for drug residues in plant-based foods. The study was published in the internationally renowned journal Frontiers in Environmental Science.

The presence of drug residues in commercially sold fruit and vegetables has already been scientifically investigated many times. However, chemical substances from tire wear, so-called additives, also find their way into the food chain. This has now been shown in a new study by an international research team led by Thilo Hofmann at the Center for Microbiology and Environmental Systems Science at the University of Vienna (CeMESS) in collaboration with a team the Hebrew University of Jerusalem led by Benny Chefetz. Vegetables from Switzerland and Israel were examined. Some of these substances and their transformation products can potentially pose ecological and toxicological risks.

Car tires consist of a complex mixture of materials that improve their performance and durability. These include 5-15% chemical additives, which comprise hundreds of substances, for example antioxidants, antiozonants, vulcanizing agents, anti-aging agents and many more, to enable the hig-tech performance of a modern tire. "The toxicity of tire and road wear particles is related to their organic additives and associated transformation products," explains Anya Sherman, PhD student at CeMESS and first author of the recently published study.

The compounds extracted from car tires find their way into agriculture through atmospheric deposition, irrigation with treated wastewater and the use of sewage sludge as fertilizer. "There they can be taken-up by plants and thus also reach humans," adds Thilo Hofmann, head of the research group.

Residues of tire wear in leafy vegetables from the supermarket and field

Finally, the researchers extrapolated the measured values from the vegetables to the intake of these substances in the diet. "We calculated the intake per day based on what people in Switzerland and Israel eat," says Sherman. The concentrations of the tire additives in leafy vegetables are low overall and are, for example, 238 ng/kg for benzothiazole (BTZ), or 0.4 ng/kg for 6PPD, a substance whose transformation product 6PPD quinone is known to be highly toxic for aquatic species like coho salmon. Depending on the diet, this leads to a daily intake per person of 12 to 1,296 ng for BTZ, or 0.06 to 2.6 ng for 6PPD. This is comparable in magnitude to drug residues, which also enter the food chain. According to Thilo Hofmann, the study shows clear results: "While the concentrations and daily intake are fortunately relatively low, additives from car tires are still found in food. That's not where they belong." According to Hofmann, the next steps should now be to investigate the environmental and human health aspects. 

From the street, to the plant, into the body

As early as 2023, the scientists were able to show that additives from car tires can in principle be absorbed by plants. "However, the question was whether this only happens in our mechanistic laboratory study or also in the field," explains first author Anya Sherman. In the current study, the Viennese and Israeli environmental scientists therefore analyzed whether lettuce plants absorb the chemicals released by car tires under natural growing conditions. "We examined real samples from supermarkets in Switzerland and field vegetables from Israel," says Thilo Hofmann, explaining the background to the study published last week.

The international team of researchers used high-resolution mass spectrometry to analyze the samples for a total of sixteen tire-associated compounds. The countries of origin of the leafy vegetables in the Swiss samples from the supermarket were Italy, Spain, and Switzerland. In the Israeli samples, field vegetables from Israel directly after harvest.

Original publication:

"Uptake of Tire-Derived Compounds in Leafy Vegetables and Implications for Human Dietary Exposure", Anya Sherman, Luzian E. Hämmerle, Evyatar Ben Mordechay, Benny Chefetz, Thorsten Hüffer, Thilo Hofmann; Frontiers in Environmental Science (2024)
DOI: 10.3389/fenvs.2024.1384506 

More information on current research at the University of Vienna can be found in the University of Vienna's science magazine Rudolphina in the general section Nature, Climate and the Cosmos.

Picture:

Fig. 1: The "Setup Lab" shows the first step of the sample processing. The samples were purchased in grocery stores in Switzerland, then shipped frozen to the scientists. They were first freeze-dried (to remove water), and then extracted in the laboratory, before measuring them with liquid chromatography-mass spectrometry. C: Anya Sherman / Center for Microbiology and Environmental Systems Science